New generation QuIC assays for prion seeding activity
نویسندگان
چکیده
منابع مشابه
Factors That Improve RT-QuIC Detection of Prion Seeding Activity
Rapid and sensitive detection of prions is important in managing prion diseases. The real-time quaking-induced conversion (RT-QuIC) assay for prion seeding activity has been applied to many prion diseases and provides for specific antemortem diagnostic testing. We evaluated RT-QuIC's long-term consistency and varied multiple reaction parameters. Repeated assays of a single scrapie sample using ...
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* Limit of detection < 100 amol of biotinylated and phosphorylated polypeptide (P-Tyr-100 assay kit, PerkinElmer, #6760620C), measured in white 384 small volume microplates (17 μL/well) Limit of detection was calculated according to the IUPAC standard: 3x(SDblank)/slope ©2016 All rights reserved. All logos and trademarks are the property of BMG LABTECH. Detection Modes Fluorescence Intensity in...
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A major problem for the effective diagnosis and management of prion diseases is the lack of rapid high-throughput assays to measure low levels of prions. Such measurements have typically required prolonged bioassays in animals. Highly sensitive, but generally non-quantitative, prion detection methods have been developed based on prions' ability to seed the conversion of normally soluble proteas...
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Different transmissible spongiform encephalopathy (TSE)-associated forms of prion protein (e.g. PrP(Sc)) can vary markedly in ultrastructure and biochemical characteristics, but each is propagated in the host. PrP(Sc) propagation involves conversion from its normal isoform, PrP(C), by a seeded or templated polymerization mechanism. Such a mechanism is also the basis of the RT-QuIC and eQuIC pri...
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ژورنال
عنوان ژورنال: Prion
سال: 2012
ISSN: 1933-6896,1933-690X
DOI: 10.4161/pri.19430